Stress-induced changes in immune signatures in ME/CFS patients determined by transcriptome analysis
Authors
Jackson Gamer, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Derek Van Booven, Dr. J.P. Hussman Institute for Human Genomics, Miller School of Medicine, University of Miami, Miami, FL, USA.
Oskar Zarnowski, Advocate Health - MidwestFollow
Melanie Perez, Department of Emergency Medicine, University of Texas Southwestern, Dallas, TX, USA.
Joshua Frank, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Rajendra P. Pangeni, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Fanny Collado, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Nancy G. Klimas, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
Elisa Oltra, School Medicine, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain.
Lubov Nathanson, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA. LNathanson@nova.edu.
Recommended Citation
Gamer J, Van Booven D, Zarnowski O, et al. Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis. In: Walker J, ed. Methods in Molecular Biology. Springer International Publishing; 2025;2920:103-112. doi:10.1007/978-1-0716-4498-0_7
Abstract
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic, complex multi-organ illness characterized by unexplained debilitating fatigue and post-exertional malaise (PEM). We evaluated transcriptomic changes in peripheral blood mononuclear cells (PBMC) of ME/CFS patients undergoing an exercise challenge and explored the transcriptomic response to exercise and recovery in PBMC of ME/CFS patients, as compared to healthy controls using RNA sequencing technology. As transcriptomic changes in ME/CFS patients are still in the phase of discovery, analysis of data has to be stringent, and the most important results have to be validated by a different technology, such as real-time PCR or NanoString.
Book Chapter/Book Details
In: Walker J, ed. Methods in Molecular Biology. Springer International Publishing; 2025.
Affiliations
Advocate Lutheran General Hospital