Stress-induced changes in immune signatures in ME/CFS patients determined by transcriptome analysis

Authors

• Jackson Gamer, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
• Derek Van Booven, Dr. J.P. Hussman Institute for Human Genomics, Miller School of Medicine, University of Miami, Miami, FL, USA.
• Oskar Zarnowski, Advocate Health - MidwestFollow
• Melanie Perez, Department of Emergency Medicine, University of Texas Southwestern, Dallas, TX, USA.
• Joshua Frank, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
• Rajendra P. Pangeni, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
• Fanny Collado, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
• Nancy G. Klimas, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA.
• Elisa Oltra, School Medicine, Universidad Católica de Valencia San Vicente Mártir, Valencia, Spain.
• Lubov Nathanson, Institute for Neuro Immune Medicine, Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Fort Lauderdale, FL, USA. LNathanson@nova.edu.

Recommended Citation

Gamer J, Van Booven D, Zarnowski O, et al. Stress-Induced Changes in Immune Signatures in ME/CFS Patients Determined by Transcriptome Analysis. In: Walker J, ed. Methods in Molecular Biology. Springer International Publishing; 2025;2920:103-112. doi:10.1007/978-1-0716-4498-0_7

Affiliations

Advocate Lutheran General Hospital

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic, complex multi-organ illness characterized by unexplained debilitating fatigue and post-exertional malaise (PEM). We evaluated transcriptomic changes in peripheral blood mononuclear cells (PBMC) of ME/CFS patients undergoing an exercise challenge and explored the transcriptomic response to exercise and recovery in PBMC of ME/CFS patients, as compared to healthy controls using RNA sequencing technology. As transcriptomic changes in ME/CFS patients are still in the phase of discovery, analysis of data has to be stringent, and the most important results have to be validated by a different technology, such as real-time PCR or NanoString.

Type

Book Chapter

PubMed ID

40372680

Book Chapter/Book Details

In: Walker J, ed. Methods in Molecular Biology. Springer International Publishing; 2025.